General

Cell Line
hPSCreg name	RIe007-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	RIe007-A (RRID:CVCL_9388)
Alternative name(s)	Royan H7
Cell line type	Human embryonic stem cell (hESC)
Similar lines	No similar lines found.
Last update	3rd September 2022
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Provider
Generator	Royan Institute (RI) Contact: Embryonic Stem Cell Biology
Derivation country	Iran
External Databases
Cellosaurus	CVCL_9388
Wikidata	Q54951007
General Information
Publications	Taei A et al. Derivation of new human embryonic stem cell lines from preimplantation genetic screening and diagnosis-analyzed embryos. In vitro cellular & developmental biology. Animal. 2010 Apr;46(3-4):395-402.
* Is the cell line readily obtainable for third parties?	Yes

Donor Information

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases
Disease associated phenotypes	chromosomal abnormalities

Ethics

Alternatives to consent are available?	Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	National
Approval number

hESC Derivation

Supernumerary embryos from IVF treatment?	Yes
PGD Embryo?	Yes

Culture Conditions

Feeder cells	mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040
Medium	Other medium: Base medium: Knockout-DMEM Main protein source: Fetal bovine serum Serum concentration: % Supplements Supplement Amount Unit L-Glutamine

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
Alkaline Phosphatase	Yes
POU5F1 (OCT-4)	Yes
SSEA-3	Yes
TRA 1-60	Yes
TRA 1-81	Yes
NANOG	Yes
SOX2	Yes

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vitro spontaneous differentiation

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vitro spontaneous differentiation

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vitro directed differentiation

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46XX Passage number: 25
Other Genotyping (Cell Line)