Man-2
CMFTe001-A
General
Cell Line |
|
| hPSCreg name | CMFTe001-A |
| Cite as: | CMFTe001-A (RRID:CVCL_B894) |
| Alternative name(s) |
Man-2
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 21st October 2019 |
| User feedback | |
Provider |
|
| Generator |
Central Manchester and Manchester Children's University Hospitals NHS (CMFT)
Contact:
IVF Unit |
| Derivation country | United Kingdom |
External Databases |
|
| Cellosaurus | CVCL_B894 |
| BioSamples | SAMEA6260503 |
| Wikidata | Q54903993 |
General Information |
|
| Publications | |
| Projects | |
| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | |
External Databases (Donor) |
|
| BioSamples | SAMEA6260504 |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | |
| How may genetic information associated with the cell line be accessed? | |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Local ehical and national legal authorization |
| Approval number |
hESC Derivation
| Date of derivation | 2007-11-13 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| Expansion status |
6
|
| ICM morphology |
A
|
| Trophectoderm morphology |
a
|
| ZP removal technique | Chemical |
| Cell isolation | Mechanical |
| Cell seeding | Isolated ICM |
Culture Conditions
| Surface coating | Gelatin | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
|
||||
| POU5F1 (OCT-4) |
Yes |
|
||||
| SSEA-3 |
Yes |
|
||||
| SSEA-4 |
Yes |
|
||||
| TRA 1-60 |
Yes |
|
||||
| TRA 1-81 |
Yes |
|
||||
| hTERT |
Yes |
|
||||
| SSEA-1 |
No |
|
Differentiation Potency
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 28
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.