DCM
MRIi010-A
General
Cell Line |
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| hPSCreg name | MRIi010-A |
| Cite as: | MRIi010-A |
| Alternative name(s) |
DCM
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 31st October 2024 |
| User feedback | |
Provider |
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| Generator |
Klinikum rechts der Isar (MRI)
Contact:
Klinikum rechts der Isar (MRI) |
| Derivation country | Germany |
External Databases |
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| BioSamples | SAMEA116330379 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclones | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
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External Databases (Donor) |
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| BioSamples | SAMEA116330380 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | Yes |
Does consent permit research by | |
| an academic institution? | Yes |
| a public organisation? | No |
| a non-profit company? | No |
| a for-profit corporation? | No |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Ethical Commission of the Faculty of Medicine (TUM) |
| Approval number | 2109/08 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
hIPSC Derivation
General |
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| Source cell type | |
| Source cell origin | |
| Collected in | 2011 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
Immunostaining, PCR
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| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Medium |
Essential 8™
Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| TRA 1-81 |
Yes |
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| SOX2 |
Yes |
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| REX1 - RNA exonuclease 1 homolog |
Yes |
Method documentation
Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| SOX7 SRY-box transcription factor 7 |
Yes |
Protocol or reference
In vitro spontaneous differentiation
| Marker | Expressed |
| ACTA2 – actin alpha 2, smooth muscle |
Yes |
Protocol or reference
In vitro spontaneous differentiation
| Marker | Expressed |
| KRT14 keratin 14 |
Yes |
| TH tyrosine hydroxylase |
Yes |
Protocol or reference
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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