Generation and characterization of a human induced pluripotent stem cell line UOWi005-A from dermal fibroblasts derived from a CCNF(S621G) familial amyotrophic lateral sclerosis patient using mRNA reprogramming
Summary
Dermal fibroblasts from a 59 year old male patient with amyotrophic lateral sclerosis (symptomatic at the time of collection), attributed to a mutation in the cyclin F gene (CCNFS621G), were reprogrammed using mRNA and microRNA-delivered OSKM factors to induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed pluripotent, expressing typical pluripotency markers and were capable of three germ layer differentiation. This is the first reported reprogramming of cells with a mutation in the cyclin F gene, and represents a novel resource for the study of amyotrophic lateral sclerosis. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Bax M, Balez R, Muñoz SS, Do-Ha D, Stevens CH, Berg T, Cabral-da-Silva MC, Engel M, Nicholson G, Yang S, Blair IP, Ooi L |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2019 Oct;40:101530 |
| PubMed | 31445393 |
| DOI | 10.1016/j.scr.2019.101530 |