RCPCMi009-A-1

IPSRG4S-206

General

Cell Line

hPSCreg name RCPCMi009-A-1
Cite as:
RCPCMi009-A-1 (RRID:CVCL_C6SD)
Alternative name(s)
IPSRG4S-206
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
RCPCMi009-A
(IPSRG4S, UEF-3B)
Last update 9th June 2023
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Provider

Generator Federal State Budgetary Institution Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency (RCPCM)

External Databases

Cellosaurus CVCL_C6SD
BioSamples SAMEA113581024
Wikidata Q117704820

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA112138836

Ethics

Also have a look at the ethics information for the parental line RCPCMi009-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line RCPCMi009-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR
Notes on reprogramming vector detection UEF-3B on the picture

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SOX2
Yes
SALL-4
Yes
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
TRA 1-81
Yes
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
Differentiation Potency
Hepatocyte
Ont Id: CL_0000182
In vitro spontaneous differentiation
Marker Expressed
HNF3b
Yes
Morphology
Endothelial Cell
Ont Id: NCIT_C12865
Marker Expressed
Vimentin
Yes
Morphology
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
tubulin beta 3
Yes
Morphology

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: 49
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Knockout of gene UBE2A
Synonyms
  • X-linked intellectual disability-nail dystrophy-seizures syndrome
Genetic modifications
UBE2A (target)
Gene knock-out
CRISPR-associated (CRISPR/Cas) System