Generation and characterization of human induced pluripotent stem cell (hiPSC) lines from an Alzheimer's disease (ASUi003-A) and non-demented control (ASUi004-A) patient homozygous for the Apolipoprotein e4 (APOE4) risk variant
Summary
Although the majority of late-onset Alzheimer's disease (AD) patients are labeled sporadic, multiple genetic risk variants have been identified, the most powerful and prevalent of which is the e4 variant of the Apolipoprotein E (APOE) gene. Here, we generated human induced pluripotent stem cell (hiPSC) lines from the peripheral blood mononuclear cells (PBMCs) of a clinically diagnosed AD patient [ASUi003-A] and a non-demented control (NDC) patient [ASUi004-A] homozygous for the APOE4 risk allele. These hiPSCs maintained their original genotype, expressed pluripotency markers, exhibited a normal karyotype, and retained the ability to differentiate into cells representative of the three germ layers. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Brookhouser N, Zhang P, Caselli R, Kim JJ, Brafman DA |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2017 Dec;25:266-269 |
| PubMed | 29246571 |
| DOI | 10.1016/j.scr.2017.07.003 |