Generation of two human induced pluripotent stem cell lines with BAX and BAK1 double knock-out using CRISPR/Cas9

Summary

Bcl-2-associated X protein (BAX) and Blc-2 homologous antagonist killer 1 (BAK) are two pro-apoptotic members of BCL2 family. Here, two BAX/BAK double knock-out human induced pluripotent stem cell lines (iPSC) we generated using CRISPR-Cas9 to generate apoptosis incompetent cell lines. The resulting cell lines were karyotypically normal, had typical morphology and expressed typical markers for the undifferentiated state. Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Ludwik KA, Hellwig L, Fisch T, Contzen J, Schaar C, Mergenthaler P, Stachelscheid H
Journal Stem cell research
Publication Date 2024 Apr;76:103377
PubMed 38460306
DOI 10.1016/j.scr.2024.103377

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