Generation of two human induced pluripotent stem cell lines with BAX and BAK1 double knock-out using CRISPR/Cas9
Summary
Bcl-2-associated X protein (BAX) and Blc-2 homologous antagonist killer 1 (BAK) are two pro-apoptotic members of BCL2 family. Here, two BAX/BAK double knock-out human induced pluripotent stem cell lines (iPSC) we generated using CRISPR-Cas9 to generate apoptosis incompetent cell lines. The resulting cell lines were karyotypically normal, had typical morphology and expressed typical markers for the undifferentiated state. Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Ludwik KA, Hellwig L, Fisch T, Contzen J, Schaar C, Mergenthaler P, Stachelscheid H |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Apr;76:103377 |
| PubMed | 38460306 |
| DOI | 10.1016/j.scr.2024.103377 |