General

Cell Line

hPSCreg Name
KUMi004-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 17th January 2022
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Provider

Generator Korea University Medical School Hospital (KUM)
Owner Korea University Medical School Hospital (KUM)
Distributors
Derivation country South Korea

External Databases

BioSamples SAMEA12063014
CLO CLO_0103713

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female
Age of donor (at collection) 65-69
Ethnicity Korean

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Chronic Lymphocytic Leukemia
The donor is a carrier of a disease-associated mutation.
Synonyms
  • CLL
  • B-Cell Chronic Lymphocytic Leukemia
  • B Cell Lymphocytic Leukemia
  • Chronic lymphocytic leukemia, NOS
  • Hematopoeitic - Chronic Lymphocytic Leukemia (CLL)
  • B-Cell Chronic Lymphoid Leukemia
  • B Cell CLL
  • Chronic Lymphocytic Leukemia (CLL)
  • Chronic Lymphocytic Leukemia
  • Chronic lymphocytic leukemia
  • chronic lymphocytic leukemia
  • Chronic B-Cell Lymphocytic Leukemia
  • B-Cell Lymphocytic Leukemia
  • B Cell Chronic Lymphocytic Leukemia
  • B-Cell CLL
  • BCLL
  • Chronic lymphatic leukemia
show more synonyms
Disease associated phenotypes no phenotypes
Family history NA
Is the medical history available upon request? NA
Is clinical information available? Yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA12063015

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? Yes
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? Yes
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided: access anonymized medical record
Does consent permit access to any other source of information about the clinical treatment or health of the donor? Yes
Contact data, institution, or website: access anonymized medical record
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? commercial kits used (Sendai Vrius)
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

Source cell line name CLL_CD34+_iPSCs
Source cell type
Hematopoietic Progenitor Cell Antigen CD34
Hematopoietic progenitor cell antigen CD34 (385 aa, ~41 kDa) is encoded by the human CD34 gene. This protein plays a role in cell-cell adhesion and may have a role in leukocyte migration.
Synonyms
  • HPCA1
  • CD34 antigen
  • gp105-120
  • Hematopoietic Progenitor Cell Antigen CD34
  • CD34
show more synonyms
Source cell origin
Hematopoietic Stem Cell
Primitive blood cells derived from embryonic mesenchyme capable of differentiating into any of the blood cell line progenitor cells (erythroblasts, young granulocytic series cells, megakaryocytes, etc.)
Synonyms
  • Hematopoietic Progenitor Cell
  • Ferrata cell
  • Hemohistioblast
  • Hematohistioblast
  • Hematopoietic Stem Cell
  • HSC
  • HSCs
  • HPC
  • Hematopoietic Progenitor Cells
  • Hematopoietic Stem Cells
show more synonyms
Age of donor (at collection) 65-69
Collected in 2021
Passage number reprogrammed 6

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Mechanically
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
SSEA-4
Yes
SOX2
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Morphology

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX t(13;14)(q32;q11.2q24.3)
Passage number: 6
Karyotyping method: G-Banding

Other Genotyping (Cell Line)