AG08C5

General

Cell Line

hPSCreg name PGNMi001-A
Cite as:
PGNMi001-A (RRID:CVCL_D0KP)
Alternative name(s)
AG08C5
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 16th November 2023
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Provider

Generator Institut NeuroMyoGene - Pathophysiology and Genetics of Neuron and Muscle (INMG-PGNM) (PGNM)
Distributors
Derivation country France

External Databases

BioSamples SAMEA112891307
Cellosaurus CVCL_D0KP
Wikidata Q123033268

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclones

Donor Information

General Donor Information

Sex male
Age of donor (at collection) 1-4
Ethnicity Asian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history hiPSC derived from healthy male donor AG08498 fibroblast cells (https://catalog.coriell.org/0/Sections/Search/Sample_Detail.aspx?Ref=AG08498&Product=CC)

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46, XY

External Databases (Donor)

BioSamples SAMN03120540

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Coriell
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information Coriell: https://www.coriell.org/0/Sections/Support/NIA/Model.aspx?PgId=351
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell line name AG08498
Source cell type
Synonyms
  • fibroblast
  • Fibroblasts
  • Fibroblast
  • FIBROBLAST
show more synonyms
Source cell origin
Age of donor (at collection) 1-4
Collected in 1985

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Genes
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Selection criteria for clones HiPS colonies were picked about 4 to 6 weeks after infection based on ES cell-like morphology.
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
TrypLE
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemMACS iPS-Brew XF (Miltenyi)
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
NANOG
Yes
SOX2
Yes
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
Cell morphology of the clone, 4 days after thawing and grown in StemMACSTM iPS Brew XF Medium (Miltenyi Biotec)
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
FOXA2
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
TBXT
Yes
HAND1
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
MAP2
Yes
PAX6
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: 34
Karyotyping method: G-Banding

Other Genotyping (Cell Line)