Royan H5
RIe005-A
General
Cell Line |
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hPSCreg Name | RIe005-A |
Alternative name(s) |
Royan H5
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Cell line type | Human embryonic stem cell (hESC) |
Last update | 28th July 2017 |
User feedback | |
Provider |
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Generator |
Royan Institute (RI)
Contact:
Embryonic Stem Cell Biology |
Derivation country | Iran |
External Databases |
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Cellosaurus | CVCL_9386 |
CLO | CLO_0100242 |
Wikidata | Q54950988 |
General Information |
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Publications |
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* Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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Sex | female |
Phenotype and Disease related information (Donor) |
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Diseases |
Ethics
Alternatives to consent are available? | Yes |
Alternatives to consent |
hESC Derivation
Embryo stage | Blastula with ICM and Trophoblast |
Supernumerary embryos from IVF treatment? |
Yes
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PGD Embryo? |
No |
Expansion status |
4![]() |
ICM morphology |
A![]() |
Trophectoderm morphology |
a![]() |
ZP removal technique | Chemical |
Cell isolation | Immunosurgery |
Cell seeding | Isolated ICM |
Culture Conditions
Surface coating | Gelatin | |||||||||
Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
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Passage method | Enzymatically and Mechanically | |||||||||
Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Fetal Calf Serum Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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Alkaline Phosphatase |
Yes |
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nodal |
Yes |
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ZFP42 (REX-1) |
Yes |
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LEFTY1 |
Yes |
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foxd3 |
Yes |
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CXCR4 |
Yes |
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EPHA2 |
Yes |
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FGF4 |
Yes |
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NANOG |
Yes |
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PAX6 |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SOX2 |
Yes |
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SSEA-1 |
No |
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TDGF1 |
Yes |
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Thy-1 |
Yes |
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PROML1 (AC133) |
Yes |
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THY1 (CD90) |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46XX
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Other Genotyping (Cell Line) |
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