Royan H5
RIe005-A
General
Donor Information
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| Expansion status |
4
|
| ICM morphology |
A
|
| Trophectoderm morphology |
a
|
| ZP removal technique | Chemical |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
Culture Conditions
| Surface coating | Gelatin | |||||||||
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
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| Passage method | Enzymatically and Mechanically | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Fetal Calf Serum Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| Alkaline Phosphatase |
Yes |
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| nodal |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| LEFTY1 |
Yes |
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| foxd3 |
Yes |
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| CXCR4 |
Yes |
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| EPHA2 |
Yes |
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| FGF4 |
Yes |
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| NANOG |
Yes |
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| PAX6 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| SSEA-1 |
No |
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| TDGF1 |
Yes |
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| Thy-1 |
Yes |
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| PROML1 (AC133) |
Yes |
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| THY1 (CD90) |
Yes |
|
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XX
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Other Genotyping (Cell Line) |
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