Immature mDA neurons ameliorate motor deficits in a 6-OHDA Parkinson's disease mouse model and are functional after cryopreservation


Parkinson's disease is associated with the loss of dopaminergic neurons in the midbrain. Clinical studies investigating replacement of these neurons with in vitro-generated neurons are currently underway. However, this approach has been limited by difficulties in scaling up on-demand production of midbrain dopaminergic (mDA) neurons from pluripotent stem cells. Cryo-preservation may offer a solution, as it allows for banking of quality controlled mDA neurons. In this study, we tested different freezing conditions and found that optimal cryopreservation of immature human mDA neurons at an early differentiation time point was achieved in STEM-CELLBANKER medium using a controlled freezing program. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Leitner D, Ramamoorthy M, Dejosez M, Zwaka TP
Journal Stem cell research
Publication Date 2019 Dec;41:101617
PubMed 31731178
DOI 10.1016/j.scr.2019.101617

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