Generation of induced pluripotent stem cell GZHMCi002-A from peripheral blood mononuclear cells with APOB mutation
Summary
Apolipoprotein (apo) B is a large, amphipathic glycoprotein which plays an important role in human lipoprotein metabolism. The 43-kb APOB gene located on the short arm of human chromosome 2 and consisted of 29 exons, mutations in the APOB gene can give rise to either hypo- or hypercholesterolemia. We used peripheral blood mononuclear cells (PBMCs) from a volunteer carrying the APOB mutation (c.10579C>T, p.Arg3527Trp) located in exon 9 to establish induced pluripotent stem cells (iPSC), which will be an effective means to reveal the key biologically relevant metabolic mechanisms, a powerful tool for medicine selection and related research. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Bing S, Nengqing L, Yi C, Dian L, Diyu C, Yingjun X, Lina H, Yinghong Y, Hongmei G, Xiaofang S |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2020 Dec;49:102054 |
| PubMed | 33128957 |
| DOI | 10.1016/j.scr.2020.102054 |