BCRTi001-A

P01-1

General#

Cell Line

hPSCreg Name BCRTi001-A
Alternative name(s)
P01-1
Cell line type Human induced pluripotent stem stell (hiPSC)
Last update 28th February 2017

Provider

Generator Berlin-Brandenburg Center for Regenerative Therapies (BCRT)
Owner Berlin-Brandenburg Center for Regenerative Therapies
Distributors Berlin Institute of Health
Derivation country Germany

General Information

Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
fibrodysplasia ossificans progressiva
The donor is affected.
Synonyms
  • myositis ossificans progressiva
  • progressive myositis ossificans
  • progressive ossifying myositis
  • Stone Man Syndrome
show more synonyms
Disease variants
ACVR1 (target)
2q24.1
Heterozygous
27345784

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XY

Ethics#

We are currently revising the public data view for cell lines registered in hPSCreg. This section will be visible again in the next weeks.

hIPSC Derivation#

General

Primary cell type cells isolated from urine
Developmental stage primary cell Adult
Age of donor at collection 45-49
Location of primary tissue procurement urine

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
FOXA2
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
T
Yes
MIXL1
Yes
goosecoid
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
Sox1
Yes
NEUROD1
Yes
OTX2
Yes
PAX6
Yes
Nestin
Yes

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
No

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Karyotyping method: KaryoLite™ BoBs™

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes

Genetic Modification#

Genetic Modifications (other than for reprogramming)