P01-1

General#

Cell Line

hPSCreg Name BCRTi001-A
Alternative name(s)
P01-1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator Berlin-Brandenburg Center for Regenerative Therapies (BCRT)
Owner Berlin-Brandenburg Center for Regenerative Therapies (BCRT)
Distributors
Derivation country Germany

External Databases

Cellosaurus CVCL_9S03

General Information

Publications
Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
fibrodysplasia ossificans progressiva
The donor is affected.
Synonyms
  • myositis ossificans progressiva
  • progressive myositis ossificans
  • progressive ossifying myositis
  • Stone Man Syndrome
show more synonyms
Genetic variants
ACVR1 (target)
2q24.1
Heterozygous
27345784

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XY

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell origin
urine
Excretion that is the output of a kidney
Age of donor (at collection) 45-49

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
FOXA2
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
T
Yes
MIXL1
Yes
goosecoid
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
Sox1
Yes
NEUROD1
Yes
OTX2
Yes
PAX6
Yes
Nestin
Yes

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Karyotyping method: KaryoLite™ BoBs™

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes