HPSI1113i-qolg_1

General#

Cell Line

hPSCreg Name WTSIi004-B
Alternative name(s)
HPSI1113i-qolg_1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 9th May 2018
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors
Derivation country United Kingdom

External Databases

BioSamples SAMEA2464797
HipSci HPSI1113i-qolg_1
Cellosaurus CVCL_AI01
ECACC 77650145
EBiSC WTSIi004-B

General Information

Publications
Projects
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2398014
HipSci HPSI-qolg

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 35-39
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score Link to microarray data
54.554 0.861 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 21

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI1113i-qolg_1
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127413
Imputed and phased genotypes
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1203461
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266800
Imputed and phased genotypes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203421
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203437
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266896
BWA alignment
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ267020
Abundances of transcripts
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127648
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1243464
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1882666
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266706
mpileup variant calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ266953
Splice-aware STAR alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203389
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448057
GATK haplotype calls