SCTCi017-A-1

General

Cell Line

hPSCreg Name
SCTCi017-A-1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 20th June 2023
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Provider

Generator Radboudumc - Department of Human Genetics (RMCGEN)

External Databases

BioSamples SAMEA113690252

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Stargardt disease
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Fundus flavimaculatus
  • Stargardt 1

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA11422973

Ethics

Also have a look at the ethics information for the parental line SCTCi017-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line SCTCi017-A.

Reprogramming method

Vector type Integrating
Vector Virus ()
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium Essential 8™ Flex
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
SOX2
Yes
SSEA-4
Yes
SSEA-4
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Stargardt Disease
Synonyms
  • Stargardt Disease
Genetic modifications
ABCA4 (target)
Isogenic modification
Chr1
NM_000350_c.5196+1137A>G
Heterozygous
Repaired