Generation of induced pluripotent stem cells from a patient with Parkinson's disease carrying LRRK2 p.I2012T mutation
Parkinson's disease (PD) is a progressive neurodegenerative disorder caused by interactions between genetic and environmental factors. Leucine rich repeat kinase (LRRK2) is the most prevalent mutation in autosomal-dominant inheritance of PD. Here, we generated induced pluripotent stem cells (iPSCs) from the peripheral blood mononuclear cells of a female patient with p.I2012T mutation in LRRK2 gene by using the Sendai-virus delivery system. The resulting iPSCs had a normal karyotype. The iPSCs also showed pluripotency confirmed by immunofluorescent staining and differentiated into the 3 germ layers in vivo. This cellular model will provide a useful platform for further pathophysiological studies of PD. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.
|Authors||Lin CH, Cheng YC, Lin HI, Ho MC, Hsu YH, Wen CH, Ko HW, Lu HE, Huang CY, Hsieh PCH|
|Journal||Stem cell research|
|Publication Date||2017 Dec;25:123-127|