Generation of a PDGFRB-mCherry knock-in reporter human induced pluripotent stem cell line (KITi001-A-1), using CRISPR/Cas9 nuclease


PDGFRB encodes platelet-derived growth factor receptor beta (PDGFR-β), a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. It is required for the normal development of the vascular and nervous systems and rearrangement of the actin cytoskeleton. PDGFR-β plays an essential role in early liver diseases, including liver fibrosis. Here, we generated a human induced pluripotent stem cell (iPSC) line, KITi001-A-1, using CRISPR/Cas9. This reporter iPSC line and its derivatives are useful for tracing PDGFR-β-expressing cells and for screening for liver fibrosis-inducing compounds. Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Jo S, Kim JW, Noh H, Kim H, Kim JH, Park HJ
Journal Stem cell research
Publication Date 2023 Jun;69:103081
PubMed 37001365
DOI 10.1016/j.scr.2023.103081

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