Generation of a PDGFRB-mCherry knock-in reporter human induced pluripotent stem cell line (KITi001-A-1), using CRISPR/Cas9 nuclease
Summary
PDGFRB encodes platelet-derived growth factor receptor beta (PDGFR-β), a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. It is required for the normal development of the vascular and nervous systems and rearrangement of the actin cytoskeleton. PDGFR-β plays an essential role in early liver diseases, including liver fibrosis. Here, we generated a human induced pluripotent stem cell (iPSC) line, KITi001-A-1, using CRISPR/Cas9. This reporter iPSC line and its derivatives are useful for tracing PDGFR-β-expressing cells and for screening for liver fibrosis-inducing compounds. Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Jo S, Kim JW, Noh H, Kim H, Kim JH, Park HJ |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2023 Jun;69:103081 |
| PubMed | 37001365 |
| DOI | 10.1016/j.scr.2023.103081 |