General

Cell Line
hPSCreg name	DVSi001-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	DVSi001-A (RRID:CVCL_D0ES)
Alternative name(s)	KRAS-Q70L-iPSC
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	No similar lines found.
Last update	14th December 2023
User feedback show/hide	Written by on Delete No feedback available yet. Login to share your feedback, experiences or results with the research community.
Provider
Generator	Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University (DVS)
External Databases
BioSamples	SAMEA114323041
Cellosaurus	CVCL_D0ES
Wikidata	Q123031194
General Information
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: not allowed Commercial use: not allowed

Donor Information

General Donor Information
Sex	male
Ethnicity	Han chinese
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Varicose Veins The donor is affected.
Family history	Unknown
Karyotyping (Donor)
Has the donor karyotype been analysed?	Yes 46, XY Karyotyping method: G-Banding
Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?	No
External Databases (Donor)
BioSamples	SAMEA114604949

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived?	Yes
Was the consent voluntarily given?	Yes
Has the donor been informed that participation will not directly influence their personal treatment?	Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor?	Yes
Do you (Depositor/Provider) hold the original Donor Consent Form?	Yes
Alternatives to consent are available?	No
Is there other documentation provided to the donor for consenting purposes?	No
Confirm that consent was obtained by a qualified professional	Yes
Has the donor agreed to be re-contacted?	No
Has the donor been informed about how her/his data will be protected?	Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised.	anonymised
Does consent explicitly allow the derivation of pluripotent stem cells?	Yes
Does consent expressly prevent development of commercial products?	No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it?	No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time?	Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time?	No
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world?	No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world?	No
Does consent permit research by
an academic institution?	Yes
a public organisation?	No
a non-profit company?	Yes
a for-profit corporation?	No
How may genetic information associated with the cell line be accessed?	Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample?	No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	The Second Affiliated Hospital of Soochow University
Approval number	No.2020-DVS256
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
Source cell type	Peripheral Blood Mononuclear Cell A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes. Synonyms PERIPHERAL BLOOD MONONUCLEAR CELL Peripheral Blood Mononuclear Cell PBMC
Reprogramming method
Vector type	Integrating
Vector	Plasmid
Is the used vector excisable?	Unknown
Absence of reprogramming vector(s)?	Unknown
Reprogramming vectors silenced?
Vector free reprogramming
Other
Derived under xeno-free conditions	Yes
Derived under GMP?	No
Available as clinical grade?	No

Culture Conditions

Surface coating	Matrigel/Geltrex
Feeder cells	No
Passage method	Enzyme-free cell dissociation EDTA
O2 Concentration	21 %
CO2 Concentration	5 %
Medium	mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	Yes

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
NANOG	Yes

hPSC Scorecard™

Self-renewal

Positive

Endoderm

Positive

Mesoderm

Positive

Ectoderm score

Positive

Differentiation Potency

Endoderm

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46,XY Karyotype_analysis-46-XY.TIF Karyotyping method: G-Banding
Other Genotyping (Cell Line)