Generation of a human iPSC line GIBHi002-A-2 with a dual-reporter for NKX2-5 using TALENs


The human transcription factor NKX2-5 plays an important role in cardiac formation and development, and thus it can be used for isolation of cardiomyocytes (CMs) differentiated from human pluripotent stem cells (hPSCs). Here, we knocked-in enhanced GFP (eGFP) and Pac (a puromycin resistant gene; PuroR) into the exon 1 coding region of NKX2-5 from a human iPSC line iPSN0003 using TALENs. The generated GIBHi002-A-2 enables us to monitor and optimize cardiac differentiation procedures via the cardiac progenitor cells (CPCs), as well as to isolate iPSC-derived CMs for drug screening. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Zhou M, Wei R, Jiang Y, Fu J, Liu Y, Yang B, Yu B, Lin Y, Ran X, Lai WH, Chu M, Hu Y, Yang J, Tse HF
Journal Stem cell research
Publication Date 2020 Dec 10;50:102120
PubMed 33352530
DOI 10.1016/j.scr.2020.102120

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