Generation of a human iPSC line GIBHi002-A-2 with a dual-reporter for NKX2-5 using TALENs
The human transcription factor NKX2-5 plays an important role in cardiac formation and development, and thus it can be used for isolation of cardiomyocytes (CMs) differentiated from human pluripotent stem cells (hPSCs). Here, we knocked-in enhanced GFP (eGFP) and Pac (a puromycin resistant gene; PuroR) into the exon 1 coding region of NKX2-5 from a human iPSC line iPSN0003 using TALENs. The generated GIBHi002-A-2 enables us to monitor and optimize cardiac differentiation procedures via the cardiac progenitor cells (CPCs), as well as to isolate iPSC-derived CMs for drug screening. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.
|Authors||Zhou M, Wei R, Jiang Y, Fu J, Liu Y, Yang B, Yu B, Lin Y, Ran X, Lai WH, Chu M, Hu Y, Yang J, Tse HF|
|Journal||Stem cell research|
|Publication Date||2020 Dec 10;50:102120|