RC-17, RC17

General#

Cell Line

hPSCreg Name RCe021-A
Alternative name(s)
RC-17, RC17
Cell line type Human embryonic stem cell (hESC)
Last update 28th November 2017
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Provider

Generator Roslin Cells (RC)
Owner Derivation of Clinical Grade hES Cells
Distributors
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_L206
BioSamples SAMEA104620770

General Information

Publications
Projects
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA104620769

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2011-02-12
Embryo stage Cleavage (Mitosis)
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Expansion status 4
Stephenson 2007
Trophectoderm morphology g
Stephenson 2007
ZP removal technique Manual Removal of ZP
Cell isolation Trophoectoderm and ICM isolated
Cell seeding Trophectoderm and ICM seeded onto GMP compataible feeder and xeno-free KOSR media combination
Derived under xeno-free conditions?
Yes
Derivation under GMP?
Yes
Available as clinical grade?
Yes

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells dermal fibroblast
Cellfinder Ont Id: CL_0002620
Passage method Mechanically
O2 Concentration 5 %
CO2 Concentration 5 %
Medium StemPro® hESC SFM
Supplements
BSA 25 %
bFGF
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
No
SSEA-1
No
Morphology pictures
Morphology of colonies of passages 6 and 12
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)