RC-17, RC17


Cell Line

hPSCreg name RCe021-A
Cite as:
RCe021-A (RRID:CVCL_L206)
Alternative name(s)
RC-17, RC17
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 19th January 2022
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Generator Roslin Cells (RC)
Owner Derivation of Clinical Grade hES Cells
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_L206
BioSamples SAMEA104620770
Wikidata Q54949391

General Information

* Is the cell line readily obtainable for third parties?
Research use: allowed
Clinical use: allowed
Commercial use: allowed

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA104620769


Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink Assisted Conception Unit
Alternatives to consent are available? Yes
Alternatives to consent The appended file forms part of the UK Stem Cell Bank Due Diligence which precedes physical deposition that has already been approved.
Alternative consent approval number
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Scotland A Research Ethics Committee
Approval number 07/MRE00/56

hESC Derivation

Date of derivation 2011-02-12
Embryo stage Cleavage (Mitosis)
Supernumerary embryos from IVF treatment?
Separation of research and IVF treatment?
PGD Embryo?
Expansion status 4
Stephenson 2007
Trophectoderm morphology g
Stephenson 2007
ZP removal technique Manual Removal of ZP
Cell isolation Trophoectoderm and ICM isolated
Cell seeding Trophectoderm and ICM seeded onto GMP compataible feeder and xeno-free KOSR media combination
Derived under xeno-free conditions?
Derivation under GMP?
Available as clinical grade?

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells dermal fibroblast
Cellfinder Ont Id: CL_0002620
Passage method Mechanically
O2 Concentration 5 %
CO2 Concentration 5 %
Medium StemPro® hESC SFM
BSA 25 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?


Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
TRA 1-60
Morphology pictures
Morphology of colonies of passages 6 and 12
Differentiation Potency
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening


Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Passage number: 12

Other Genotyping (Cell Line)