RC-17, RC17

General#

Cell Line

hPSCreg Name RCe021-A
Alternative name(s)
RC-17, RC17
Cell line type Human embryonic stem cell (hESC)
Last update 28th November 2017
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Provider

Generator Roslin Cells (RC)
Owner Derivation of Clinical Grade hES Cells
Distributors
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_L206
BioSamples SAMEA104620770

General Information

Publications
Projects
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA104620769

Ethics#

Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Alternatives to consent are available? Yes
Alternatives to consent The appended file forms part of the UK Stem Cell Bank Due Diligence which precedes physical deposition that has already been approved.
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Scotland A Research Ethics Committee
Approval number 07/MRE00/56

hESC Derivation#

Date of derivation 2011-02-12
Embryo stage Cleavage (Mitosis)
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Expansion status 4
Stephenson 2007
Trophectoderm morphology g
Stephenson 2007
ZP removal technique Manual Removal of ZP
Cell isolation Trophoectoderm and ICM isolated
Cell seeding Trophectoderm and ICM seeded onto GMP compataible feeder and xeno-free KOSR media combination
Derived under xeno-free conditions?
Yes
Derivation under GMP?
Yes
Available as clinical grade?
Yes

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells dermal fibroblast
Cellfinder Ont Id: CL_0002620
Passage method Mechanically
O2 Concentration 5 %
CO2 Concentration 5 %
Medium StemPro® hESC SFM
Supplements
BSA 25 %
bFGF
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
No
SSEA-1
No
Morphology pictures
Morphology of colonies of passages 6 and 12
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)