Characterization of an induced pluripotent stem cell line (NCHi013-A) from a 5-year-old male with pulmonary atresia with intact ventricular septum and a biventricular repair
Summary
Pulmonary atresia with intact ventricular septum (PA/IVS) is a rare congenital heart defect that causes a significant decrease of blood outflow from the heart and is fatal if left untreated. iPSC line NCHi013-A was produced from peripheral blood mononuclear cells from a male child with PA/IVS using Sendai virus reprogramming. NCHi013-A displayed normal stem cell morphology, expressed markers for pluripotency, and presented ability to differentiate into cells of endoderm, ectoderm, and mesoderm lineages. The iPSC line also maintained normal karyotype, was validated for cell identity, and tested negative for transgenes and mycoplasma contamination. Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Hanley M, Alonzo M, Ye S, Yu Y, Contreras J, Hayden J, Garg V, Zhao MT |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Oct;80:103526 |
| PubMed | 39121650 |
| PubMed Central | PMC11404849 |
| DOI | 10.1016/j.scr.2024.103526 |