hPSCreg
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FAMRCi003-A

Registration Summary:

108-1

FAMRCi003-A

General#

Cell Line
hPSCreg Name FAMRCi003-A
Alternative name(s)
108-1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 22nd May 2019
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Provider
Generator Federal Almazov North-West Medical Research Centre (FAMRC)
Owner Federal Almazov North-West Medical Research Centre (FAMRC)
Derivation country Russia

External Databases
BioSamples SAMEA104626792
Cellosaurus CVCL_VD63

General Information
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed

Donor Information#

General Donor Information
Sex female
Age of donor (at collection) 35-39
Ethnicity Caucasian

Phenotype and Disease related information (Donor)
Diseases No disease was diagnosed.

Karyotyping (Donor)
Has the donor karyotype been analysed?
No

Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?
No

Donor Relations
Other cell lines of this donor

External Databases (Donor)
BioSamples SAMEA104626793

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? Yes
Contact information / weblink Local ethical committee of Federal Almazov North-West Medical Research Centre
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? Yes
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent permit unforeseen future research, without further consent? Yes
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Local ethical committee of Federal Almazov North-West Medical Research Centre
Approval number №13/19.06.2014
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Local ethical committee of Federal Almazov North-West Medical Research Centre
Approval number №13/19.06.2014
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General
Source cell type
adipose tissue derived mesenchymal stem cell
adipose tissue derived mesenchymal stem cell in the OLS
A adult mesenchymal stem cell derived from adipose tisssue
Synonyms
  • human mesenchymal stem cell from adipose tissue
  • hMSC-AT
Source cell origin
adipose tissue
adipose tissue in the OLS
Portion of connective tissue composed of adipocytes enmeshed in areolar tissue
Synonyms
  • fat
  • fatty tissue
  • fat tissue
Age of donor (at collection) 35-39
Passage number reprogrammed 2

Reprogramming method
Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Gelatin
Feeder cells mouse embryonic fibroblasts
Cellfinder Ont Id: EFO_0004040
Passage method Enzymatically
Collagenase
CO2 Concentration 5 %
Medium Other medium:
Base medium: KO-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
NEAA 0.1 mM
L-Glutamine 2 mM
2-Mercaptoethanol 0.25 mM
penicillin 100 U/ml
streptomycin 100 µg/ml
bFGF 10 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
TRA 1-81
Yes
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Microbiology / Virus Screening
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)
Is there genome-wide genotyping or functional data available?
Yes