hPSCreg
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FAMRCi003-A

Registration Summary:

108-iPSCs

FAMRCi003-A

General#

Cell Line
hPSCreg Name FAMRCi003-A
Alternative name(s)
108-iPSCs
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 25th December 2017
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Provider
Generator Federal Almazov North-West Medical Research Centre (FAMRC)
Owner Federal Almazov North-West Medical Research Centre (FAMRC)
Derivation country Russia

External Databases
BioSamples SAMEA104626792
Cellosaurus CVCL_VD63

General Information
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information
Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)
Diseases No disease was diagnosed.

Karyotyping (Donor)
Has the donor karyotype been analysed?
No

Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?
No

Donor Relations
Other cell lines of this donor

External Databases (Donor)
BioSamples SAMEA104626793

Ethics#

We are currently revising this section.

hIPSC Derivation#

General
Source cell type
adipose tissue derived mesenchymal stem cell
adipose tissue derived mesenchymal stem cell in the OLS
A adult mesenchymal stem cell derived from adipose tisssue
Synonyms
  • human mesenchymal stem cell from adipose tissue
  • hMSC-AT
Source cell origin
adipose tissue
adipose tissue in the OLS
Portion of connective tissue composed of adipocytes enmeshed in areolar tissue
Synonyms
  • fat
  • fatty tissue
  • fat tissue
Age of donor (at collection) 35-39
Passage number reprogrammed 2

Reprogramming method
Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Gelatin
Feeder cells mouse embryonic fibroblasts
Cellfinder Ont Id: EFO_0004040
Passage method Enzymatically
Collagenase
CO2 Concentration 5 %
Medium Other medium:
Base medium: KO-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
NEAA 0.1 mM
L-Glutamine 2 mM
2-Mercaptoethanol 0.25 mM
penicillin 100 U/ml
streptomycin 100 µg/ml
bFGF 10 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
TRA 1-81
Yes
SSEA-4
Yes
SOX2
Unkown
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Microbiology / Virus Screening
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)
Is there genome-wide genotyping or functional data available?
Yes