108-1
FAMRCi003-A
General
Cell Line |
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| hPSCreg name | FAMRCi003-A |
| Cite as: | FAMRCi003-A (RRID:CVCL_VD63) |
| Alternative name(s) |
108-1
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
FAMRCi003-B (108-2) |
| Last update | 18th April 2022 |
| User feedback | |
Provider |
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| Generator | Federal Almazov North-West Medical Research Centre (FAMRC) |
| Owner | Federal Almazov North-West Medical Research Centre (FAMRC) |
| Derivation country | Russia |
External Databases |
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| BioSamples | SAMEA104626792 |
| Cellosaurus | CVCL_VD63 |
| Wikidata | Q54833244 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Donor Information
General Donor Information |
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| Sex | female |
| Age of donor (at collection) | 35-39 |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
No
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA104626793 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Please provide the contact information | Local ethical committee of Federal Almazov North-West Medical Research Centre |
| Is there other documentation provided to the donor for consenting purposes? | No |
| Confirm that consent was obtained by a qualified professional | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Does consent permit unforeseen future research, without further consent? | Yes |
| Does consent expressly permit storage of donated embryo/tissue for an unlimited time? | Yes |
| Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | Open Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Local ethical committee of Federal Almazov North-West Medical Research Centre |
| Approval number | №13/19.06.2014 |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
| Name of accrediting authority involved? | Local ethical committee of Federal Almazov North-West Medical Research Centre |
| Approval number | №13/19.06.2014 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell type |
An adult mesenchymal stem cell derived from adipose tisssue.
Synonyms
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| Source cell origin |
Portion of connective tissue composed of adipocytes enmeshed in areolar tissue.
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| Age of donor (at collection) | 35-39 |
| Passage number reprogrammed | 2 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Gelatin | |||||||||||||||||||||
| Feeder cells |
mouse embryonic fibroblasts Cellfinder Ont Id: EFO_0004040 |
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| Passage method |
Enzymatically
Collagenase
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| CO2 Concentration | 5 % | |||||||||||||||||||||
| Medium |
Other medium:
Base medium: KO-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
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| TRA 1-81 |
Yes |
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| SSEA-4 |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46,XX
Passage number: 20
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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| Is there genome-wide genotyping or functional data available? |
Yes
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