108-iPSCs
FAMRCi003-A
General#
Cell Line |
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hPSCreg Name | FAMRCi003-A |
Alternative name(s) |
108-iPSCs
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 25th December 2017 |
User feedback | |
Provider |
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Generator | Federal Almazov North-West Medical Research Centre (FAMRC) |
Owner | Federal Almazov North-West Medical Research Centre (FAMRC) |
Derivation country | Russia |
External Databases |
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BioSamples | SAMEA104626792 |
Cellosaurus | CVCL_VD63 |
General Information |
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Is the cell line available in principle? | Available with restrictions: Available for non-commercial research |
Donor Information#
General Donor Information |
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Sex | female |
Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
No
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
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BioSamples | SAMEA104626793 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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Source cell type |
adipose tissue derived mesenchymal stem cellA adult mesenchymal stem cell derived from adipose tisssue
Synonyms
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Source cell origin |
adipose tissuePortion of connective tissue composed of adipocytes enmeshed in areolar tissue
Synonyms
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Age of donor (at collection) | 35-39 |
Passage number reprogrammed | 2 |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Is reprogramming vector detectable? |
No |
Methods used |
RT-PCR
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Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Surface coating | Gelatin | |||||||||||||||||||||
Feeder cells |
mouse embryonic fibroblasts Cellfinder Ont Id: EFO_0004040 |
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Passage method |
Enzymatically
Collagenase
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CO2 Concentration | 5 % | |||||||||||||||||||||
Medium |
Other medium:
Base medium: KO-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
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Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
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Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
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NANOG |
Yes |
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TRA 1-81 |
Yes |
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SSEA-4 |
Yes |
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SOX2 |
Unkown |
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Differentiation Potency
Microbiology / Virus Screening |
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Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46,XX
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Is there genome-wide genotyping or functional data available? |
Yes
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