Generation of PARP1 gene knockout human embryonic stem cell line using CRISPR/Cas9


PARP1 encodes a chromatin-associated enzyme which responsible for post-translational poly(ADP-ribosyl)ation modification (Hsieh et al., 2017). It plays an important role in nucleotide excision repair, non-homologous end joining, DNA mismatch repair and many other DNA repair process. Also, PARP1 participates in inflammation and aging. However, its role in human embryonic stem cell biology has not been fully resolved. To clarify the function of PARP1 in human embryonic stem cells, we reported a PARP1 knockout human embryonic stem cell line, generated by CRISPR/Cas9 mediated gene targeting. This cell line shows normal karyotype, pluripotent stem cell marker expression and differentiation potential in vitro. Copyright © 2021. Published by Elsevier B.V.

Authors Li Y, Li J, Zhou T, Pan G, Huang K
Journal Stem cell research
Publication Date 2021 May;53:102288
PubMed 33740642
DOI 10.1016/j.scr.2021.102288

Research Projects

Cell Lines