In vitro neural differentiation of human embryonic stem cells


Human embryonic stem (hES) cells are seen as a source of defined neural cell population for pharmaceutical screening or transplantation and can be conducted to differentiate in vitro to mature neurons [1]. hES cells from line HS 237T (Karolinska Institutet, Huddinge, Sweden) [2] were cultured as overgrowth embryoid bodies (EB) in a serum-free medium [3] that "directed" commitment of hES cells into neural lineages and supported the viability and maturation of progenitors to terminal phenotypes. Aspects during neural differentiation 7 days after plating of EB are presented in Fig. 1. Radial glia (GFAP positive) and neurons (MAP2 and NF 200 positive) migrate from EB. HES cells readily generate neurons in vitro, but steering their differentiation into specific neuronal subtypes remains a major challenge.

Authors Nat R, Hovatta O
Journal Journal of cellular and molecular medicine
Publication Date 2004 Oct-Dec;8(4):570-1
PubMed 15601586
PubMed Central PMC6740232
DOI 10.1111/j.1582-4934.2004.tb00482.x

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