Generation of a doxycycline-inducible ETV2 expression cell line using PiggyBac transposase system


E26 transformation-specific variant 2 (ETV2), as a member of Ets family of transcription factors, plays an important role in embryonic vasculogenesis, angiogenesis and hematopoiesis. Here, based on H9 cell line and PiggyBac technology, we generated a doxycycline-inducible ETV2 (Dox-ETV2-H9) embryonic stem cell (ESC) line. The Dox-ETV2-H9 cells maintain normal morphology, karyotype as well as high expression of pluripotent markers. In comparison with previous studies that employing costly ETV2 modRNA to improve differentiation from ESCs into endothelial cells (ECs), the tet-on system in Dox-ETV2-H9 cells could precisely manipulate ETV2 expression, which enables ECs differentiation process more efficient and controllable upon addition of doxycycline. Copyright © 2022. Published by Elsevier B.V.

Authors Zhou M, Ni J, Huang P, Liu X
Journal Stem cell research
Publication Date 2023 Feb;66:102985
PubMed 36463633
DOI 10.1016/j.scr.2022.102985

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