11713

General#

Cell Line

hPSCreg Name CDIi004-A
Alternative name(s)
11713
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 14th December 2017
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Cellular Dynamics International, a FUJIFILM Company (CDI)
Derivation country United States

External Databases

BioSamples SAMEA104626278
Cellosaurus CVCL_VE12

General Information

Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Is the medical history available upon request? No
Is clinical information available? No

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Normal
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104626279

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
blood
A fluid that is composed of blood plasma and erythrocytes.
Synonyms
  • portion of blood
  • vertebrate blood
Age of donor (at collection) 35-39

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Selection criteria for clones Clones were identified by proprietary assay to determine pluripotency.
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Feeder cells
No
Medium TeSR™ E8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
LIN28
Yes
Method documentation
Methods for hPSCreg.pdf
Methods for hIPSC characterization
Differentiation Potency

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal
Karyotyping method: G-Banding

Other Genotyping (Cell Line)