Generation of a human induced pluripotent stem cell line (MUSIi001-A) from caesarean section scar fibroblasts using Sendai viral vectors
Summary
We generated a human induced pluripotent stem cell (iPSC) line from caesarean section scar fibroblasts of a 33-year-old healthy woman using transgene-free Sendai viral vectors under feeder-free condition. The established iPSC line, designated as MUSIi001-A, exhibited a normal karyotype, expressed pluripotent markers, differentiated into cells of three embryonic germ layers. Further analyses showed that the Sendai viral genome was absent at passage 25. The MUSIi001-A line can serve as a control for studying developmental biology and phenotypic comparison with disease-specific iPSCs. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Wattanapanitch M, Ritthaphai A, Park C, Boonkaew B, Netsrithong R, Pattanapanyasat K, Limsiri P, Vatanashevanopakorn C |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2018 Mar;27:105-108 |
| PubMed | 29367084 |
| DOI | 10.1016/j.scr.2018.01.013 |