Efficient generation of induced pluripotent stem cell lines from peripheral blood mononuclear cells
Summary
Peripheral blood mononuclear cells (PBMCs) have been widely considered as a more convenient and almost unlimited reprogramming resource, while the reprogramming procedure and efficiency still need to be improved. We reprogrammed the PBMCs by using non-integrative non-viral vectors liposome electrotransfer containing the reprogramming factors OCT4, SOX2, KLF4, and c-MYC. The iPSC lines exhibited a normal karyotype with their corresponding PBMCs and exhibited significant cellular pluripotency. Teratoma formation assay revealed that the iPSCs we generated could differentiate into three embryonic germ layers. Our study provides a more effective procedure for peripheral blood monocyte reprogramming to iPSC, and promotes its future application. Copyright © 2023. Published by Elsevier B.V.
| Authors | Gao Y, Cheng Y, Wen J, Xiao D, Xu J, Cai C, Hu J |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2023 Jun;69:103088 |
| PubMed | 37099933 |
| DOI | 10.1016/j.scr.2023.103088 |