HSF-6, UC06

General#

Cell Line

hPSCreg Name UCSFe002-A
Alternative name(s)
HSF-6, UC06
Cell line type Human embryonic stem cell (hESC)
Last update 22nd November 2017
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Provider

Generator University of California, San Francisco (UCSF)
Derivation country United States

External Databases

Cellosaurus CVCL_D005

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics#

We are currently revising this section.

hESC Derivation#

Culture Conditions#

Passage method Enzymatically
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
bFGF %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-3
Yes
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
nodal
Yes
LEFTYA
Yes
EPHA2
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
THY1 (CD90)
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)