HSF-6, UC06

General

Cell Line

hPSCreg name UCSFe002-A
Cite as:
UCSFe002-A (RRID:CVCL_D005)
Alternative name(s)
HSF-6, UC06
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 30th August 2022
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Provider

Generator Conklin Lab, Gladstone/UCSF (UCSF)
Derivation country United States

External Databases

Cellosaurus CVCL_D005
Wikidata Q54896437

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics

How may genetic information associated with the cell line be accessed?

hESC Derivation

Culture Conditions

Passage method Enzymatically
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
bFGF %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SSEA-3
Yes
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
nodal
Yes
LEFTYA
Yes
EPHA2
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
THY1 (CD90)
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 43

Other Genotyping (Cell Line)