iSMA40
ICGi005-A
General#
Cell Line |
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hPSCreg Name | ICGi005-A |
Alternative name(s) |
iSMA40
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 23rd January 2019 |
User feedback | |
Provider |
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Generator | The Federal Research Center Institute of Cytology and Genetics The Siberian Branch of the Russian Academy of Sciences (ICG) |
Owner | The Federal Research Center Institute of Cytology and Genetics The Siberian Branch of the Russian Academy of Sciences (ICG) |
Distributors | |
Derivation country | Russia |
External Databases |
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BioSamples | SAMEA5100024 |
Cellosaurus | CVCL_UF65 |
General Information |
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Publications | |
Is the cell line available in principle? | Available with restrictions: Available for non-commercial research |
Donor Information#
General Donor Information |
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Sex | female |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Unkown
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
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BioSamples | SAMEA5131662 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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Source cell line name | f1SMA |
Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
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Source cell origin |
fibroblast of dermis |
Collected in | 2014 |
Passage number reprogrammed | 6 |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Episomal |
Genes | |
Is reprogramming vector detectable? |
No |
Methods used |
PCR
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Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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Other |
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Selection criteria for clones | ES-like morphology |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Surface coating | Gelatin | |||||||||||||||||||||
Feeder cells |
mouse embryonic fibroblasts Cellfinder Ont Id: EFO_0004040 |
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Passage method |
Enzymatically
TrypLE
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CO2 Concentration | 5 % | |||||||||||||||||||||
Medium |
Other medium:
Base medium: KO-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 15 % Supplements
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
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Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
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Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
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NANOG |
Yes |
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SOX2 |
Yes |
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SSEA-4 |
Yes |
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TRA 1-81 |
Yes |
Score:
Marker | Present | Absent |
mCpG | ||
OCT4 |
Morphology pictures
iSMA40.tif
Scale bar 500 µm
Differentiation Potency
In vitro spontaneous differentiation
Marker | Expressed |
CK18 |
Yes |
Morphology
isma_40_diff_KRT18_scale.tif
Scale bare 100 µm
In vitro spontaneous differentiation
Marker | Expressed |
ACTA2 |
Yes |
Morphology
isma_40_diff_a-sma_r_1_scale.tif
Scale bar 100 µm
In vitro spontaneous differentiation
Marker | Expressed |
TUBB3 |
Yes |
Morphology
isma_40_diff_bIII_200g-2_scale.tif
Scale bar 100 µm
Microbiology / Virus Screening |
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Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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