Generation of isogenic and homozygous MEN1 mutant cell lines from patient-derived iPSCs using CRISPR/Cas9
Summary
MEN1, an autosomal dominant disorder caused by mutations in the tumor suppressor gene MEN1, manifests with co-occurrence of multiple endocrine/neuroendocrine neoplasms. An iPSC line derived from an index patient carrying the mutation c.1273C>T (p.Arg465*) was edited using a single multiplex CRISPR/Cas approach to create an isogenic control non-mutated line and a homozygous double mutant line. These cell lines will be useful for elucidating subcellular MEN1 pathophysiology and for screening to identify potential MEN1 therapeutic targets. Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Even-Zohar N, Metin-Armagan D, Ben-Shlomo A, Sareen D, Melmed S |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2023 Jun;69:103124 |
| PubMed | 37209468 |
| PubMed Central | PMC10882434 |
| DOI | 10.1016/j.scr.2023.103124 |