326
VCCRIi024-A
General
iPSC Line |
|
| hPSCreg name | VCCRIi024-A |
| Cite as: | VCCRIi024-A (RRID:CVCL_E7WI) |
| Alternative name(s) |
326
|
| iPSC line type | Human induced pluripotent stem cell (hiPSC) |
| Similar iPSC lines | No similar lines found. |
| Last update | 1st July 2025 |
| User feedback | |
Provider |
|
| Generator | Victor Chang Cardiac Research Institute (VCCRI) |
| Owner | Victor Chang Cardiac Research Institute (VCCRI) |
| Distributors | |
| Derivation country | Australia |
External iPSC Databases |
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| Cellosaurus | CVCL_E7WI |
General iPSC Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Cell line can only be used in: Medical research
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:
Available upon MTA/Research agreement |
Donor Information
General Donor Information |
|
| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Is the medical history available upon request? | Yes |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| * Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| * Does consent pertain to a specific research project? | Yes |
| Details on restriction to research project | Consent as per HREC/16/SVH/338; SVH File number:16/245 |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | St Vincent's Hospital Human Research Ethics Committee |
| Approval number | (HREC/16/SVH/338); SVH File number: 16/245 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
iPSC Derivation
General |
|
| Source cell type |
A mixture of monocytes and lymphocytes; blood leucocytes from which granulocytes have been separated and removed.
Synonyms
|
| Source cell origin |
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
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Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
| Notes on reprogramming vector detection | Not detected via PCR |
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
iPSC Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at passage previously with this iPSC line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this iPSC line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this iPSC line? | Yes |
iPSC Characterisation
Analysis of undifferentiated iPSCs
Morphology pictures
Brightfield image of colony from iPSC line 326 (VCCRI024-A)
Self-renewal
Positive
Endoderm
Negative
Mesoderm
Negative
Ectoderm score
Negative
Scorecard
326 Pluripotency.pdf
Scorecard for iPSC 326
Differentiation Potency
In vitro directed differentiation
Scorecard
| Marker | Expressed |
| SOX17 |
Yes |
Morphology
Endodmeral differentiation; Scale bar 200 um; Sox17: Green; DAPI: Blue; Call out indicated nuclear and perinuclear staining Scale Bar 50 um
In vitro directed differentiation
| Marker | Expressed |
| Transgelin |
Yes |
Morphology
Transgelin (Red) and DAPI (blue) expressed in iPSC-derived vascular smooth muscle cells
Protocol or reference
132312_2023-1-30 11522_famC_ExpressionPlot.jpeg
Scorecard of Trilineage Differentiated cells
In vitro directed differentiation
Scorecard
| Marker | Expressed |
| Pax6 |
Yes |
Morphology
Ectodermal cells of a Neural Rosstte formed from an outgrowing embryoid body, 200 um; Green: Pax6; Blue: DAPI
Genotyping
Karyotyping (iPSC Line) |
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| Has the iPSC line karyotype been analysed? |
Yes
Normal Karyotype
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Other Genotyping (iPSC Line) |
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