Generation of an urine-derived induced pluripotent stem cell line from a 6-year old X-linked adrenoleukodystrophy (X-ALD) patient
Summary
The gene mutations of the ATP-binding-cassette transporter subfamily D member 1 (ABCD1) can lead to the inherited neuro-metabolic malfunction disease X-linked adrenoleukodystrophy (X-ALD). Human urine cells from a 6-year-old male X-ALD patient harboring a ABCD1 gene frameshift (c.2013insA, Xq28) were reprogrammed into the induced pluripotent stem cell (iPSC) line WMUi014-A with Sendai virus reprogramming kit containing OCT4, SOX2, c-MYC, and KLF4 Yamanaka factors. The established iPSCs in vitro stably expressed pluripotent markers, had differentiation potential into three germ layers, and maintained a normal 44 + XY karyotype. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Wang L, Gao B, Mo X, Guo X, Huang J |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 Mar;51:102170 |
| PubMed | 33472124 |
| DOI | 10.1016/j.scr.2021.102170 |