Alice, BMACA-CT2-A
PMUi001-A
General
Cell Line |
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| hPSCreg name | PMUi001-A |
| Cite as: | PMUi001-A (RRID:CVCL_C1VK) |
| Alternative name(s) |
Alice, BMACA-CT2-A
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | |
| Last update | 14th December 2021 |
| User feedback | |
Provider |
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| Generator | Paracelsus Medical University (PMU) |
External Databases |
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| BioSamples | SAMEA11496797 |
| Cellosaurus | CVCL_C1VK |
| Wikidata | Q114312664 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA11356038 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Please provide contact information of the holder of the original Donor Information Sheet. | mi.payer@medunigraz.at |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | No |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Medizinische Universität Graz |
| Approval number | 21-060 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell type |
A heterogeneous, non-hematopoietic fibroblast-like cell with the potential for differentiating into connective tissue cells of multiple lineages. It plays a significant role in tissue repair and regeneration, and interacts with immune cells to downregulate the immune response and enable immunosuppression via the induction of tolerance.
Synonyms
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| Source cell origin |
Aspirate from bone marrow.
Synonyms
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
Unknown |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
|
| O2 Concentration | 5 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Unknown |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| TRA 1-81 |
Yes |
|||||
| SSEA-4 |
Yes |
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| Oct4 |
Yes |
Differentiation Potency
In vitro directed differentiation
| Marker | Expressed |
| Thy1 |
Yes |
| 5'-nucleotidase |
Yes |
| PECAM-1 |
Yes |
| Tra-1-81 |
No |
Protocol or reference
In vitro directed differentiation
| Marker | Expressed |
| Keratin 14 |
Yes |
| Thy1 |
No |
| Tra-1-81 |
No |
Protocol or reference
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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