Chantal, BMACA-CT2-C

The cell line is not validated yet.

General

Cell Line

hPSCreg Name
PMUi001-C
Alternative name(s)
Chantal, BMACA-CT2-C
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 13th March 2022
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Provider

Generator Paracelsus Medical University (PMU)

External Databases

BioSamples SAMEA11506037
CLO CLO_0103663

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA11356038

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: mi.payer@medunigraz.at
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Medizinische Universität Graz
Approval number 21-060
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Mesenchymal Stromal Cell
A heterogeneous, non-hematopoietic fibroblast-like cell with the potential for differentiating into connective tissue cells of multiple lineages. It plays a significant role in tissue repair and regeneration, and interacts with immune cells to downregulate the immune response and enable immunosuppression via the induction of tolerance.
Synonyms
  • Mesenchymal Stromal Cell
  • MSC
Source cell origin
Bone Marrow Aspirate
Aspirate from bone marrow.
Synonyms
  • Human Bone Marrow Aspirate
  • Bone Marrow Aspirate

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
Unknown

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
O2 Concentration 5 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-81
Yes
Oct4
Yes
Differentiation Potency
Definitive Endoderm
Ont Id: UBERON_0005439
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Protocol or reference
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
CD140b
Yes
CD144
Yes
Neuroectoderm
Ont Id: NCIT_C34228
In vitro directed differentiation
Marker Expressed
SOX2
Yes
PAX6
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)