Generation of a WWTR1 mutation induced pluripotent stem cell line, MUSIi012-A-1, using CRISPR/Cas9


WWTR1 or TAZ (WWTR1/TAZ) is a transcriptional coactivator that acts as a downstream regulatory target in the Hippo signaling pathway, which plays a pivotal role in regulating cell proliferation and anti-apoptosis. It has been shown in other cell types that WWTR1/TAZ plays a redundant role to its homolog YAP1. Using CRISPR/Cas9 gene editing, we established the WWTR1/TAZ-KO cell line, which features homozygous deletion of WWTR1 gene from human iPSCs. The established WWTR1/YAZ-KO cell line maintained the pluripotent phenotype, the ability to differentiate into all three embryonic germ layers, and normal karyotype. Copyright © 2019 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Lorthongpanich C, Jiamvoraphong N, Supakun P, Damkham N, Terbto P, Waeteekul S, U-Pratya Y, Laowtammathron C, Issaragrisil S
Journal Stem cell research
Publication Date 2019 Dec;41:101634
PubMed 31677525
DOI 10.1016/j.scr.2019.101634

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