Establishment of induced pluripotent stem cell (iPSC) line from a 57-year old patient with sporadic Alzheimer's disease
Summary
Peripheral blood mononuclear cells (PBMCs) were collected from a clinically characterised 57-year old woman with sporadic Alzheimer's disease. The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system. The transgene-free iPSC showed pluripotency verified by immunocytochemistry for pluripotency markers and differentiated spontaneously towards the 3 germ layers in vitro. Furthermore, the iPSC line showed normal karyotype. Our model might offer a good platform to further study the pathomechanism of sporadic AD, to identify early biomarkers and also for drug testing and gene therapy studies. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Ochalek A, Nemes C, Varga E, Táncos Z, Kobolák J, Dinnyés A |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2016 Jul;17(1):72-74 |
| PubMed | 27558605 |
| DOI | 10.1016/j.scr.2016.05.020 |