Generation of 3 clones of induced pluripotent stem cells (iPSCs) from a patient affected by Crohn's disease


Crohn's disease is a debilitating and incurable chronic inflammatory bowel disease, affecting millions of individuals worldwide, with an increasing frequency. Surgery must be applicable in half of the cases often with a disabling course, and pharmacological treatments may have adverse complications. We generated three isogenic clones of iPSCs from peripheral blood mononuclear cells (PBMCs) of a patient with Crohn's Disease under pharmacological treatment without adverse effects. Sendai virus based vector was used and the iPSCs were characterized for genetic uniqueness, genomic integrity, pluripotency, and differentiation ability. These iPSCs will be a powerful tool to develop tailored therapies. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Lanzi G, Masneri S, Ferraro RM, Genova E, Piovani G, Barisani C, Pelin M, Stocco G, Decorti G, Bramuzzo M, Giliani S
Journal Stem cell research
Publication Date 2019 Oct;40:101548
PubMed 31536868
DOI 10.1016/j.scr.2019.101548

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