VAL-5
ESe013-A
General
Donor Information
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | |
Ethics
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | |
| How may genetic information associated with the cell line be accessed? | |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Ethics board of the Prince Felipe Research Centre/Instituto de Salud Carlos III |
| Approval number |
hESC Derivation
| Date of derivation | 2006-02-06 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| Expansion status |
3
|
| ICM morphology |
B
|
| Trophectoderm morphology |
a
|
| ZP removal technique | Chemical |
| Cell isolation | None |
| Cell seeding | Embryo |
Culture Conditions
| Feeder cells |
human foreskin fibroblast Cellfinder Ont Id: CELDA_000001419 |
|||||||||
| O2 Concentration | 21 % | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
|
||||
| NANOG |
Yes |
|
|
|||
| POU5F1 (OCT-4) |
Yes |
|
|
|||
| SSEA-4 |
Yes |
|
||||
| TRA 1-60 |
Yes |
|
||||
| TRA 1-81 |
Yes |
|
||||
| SSEA-1 |
No |
|
||||
| DNMT3B |
Yes |
|
||||
| GDF3 |
Yes |
|
||||
| ZFP42 (REX-1) |
Yes |
|
||||
| Amy |
No |
|
||||
| CR1 |
Yes |
|
||||
| GABRB3 |
Yes |
|
||||
| Lefty-A |
Yes |
|
||||
| Nfh |
No |
|
||||
| Ren |
No |
|
||||
| SOX2 |
Yes |
|
||||
| Telomerase Activity |
Yes |
|
||||
| Thy-1 |
Yes |
|
Differentiation Potency
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
Certificate of Analysis |
|
| Is there a certificate of analysis available? |
Yes
Passage:
|
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 30
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.