M2_IPSC4_P

General

Cell Line

hPSCreg name HCORDi001-I
Cite as:
HCORDi001-I (RRID:CVCL_D6IP)
Alternative name(s)
M2_IPSC4_P
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 18th May 2024
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Provider

Generator Hemocord Clínica Médica Ltda (HCORD)
Owner Hemocord Clínica Médica Ltda (HCORD)
Distributors
Derivation country Brazil

External Databases

BioSamples SAMEA115299662
Cellosaurus CVCL_D6IP

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female
Age of donor (at collection) neonate
Ethnicity White

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history Yes
Is the medical history available upon request? Yes
Is clinical information available? Yes

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
SNP typing array
Found no mutations
NGS - targeted sequencing (TS)
Expanded cancer panel containing 264 cancer-associated genes.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA114470364

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Hemocord Clínica Médica Ltda.
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Is there other documentation provided to the donor for consenting purposes? Yes
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? Yes
Details on restriction to research project Project: Development of Human Induced Pluripotent Stem Cells (IPSCs) from from Human Perinatal Tissues in vitro.
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? Yes
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? Yes
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? No
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? No
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? Yes
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? No
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided: karolyn@hemocord.com.br
Does consent permit access to any other source of information about the clinical treatment or health of the donor? Yes
Contact data, institution, or website: karolyn@hemocord.com.br
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Committee University of Vale do Rio dos Sinos (Unisinos)
Approval number 4.593.309
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Ethics Committee University of Vale do Rio dos Sinos (Unisinos)
Approval number 4.593.309
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Thermo Fisher Scientific
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

Source cell type
A heterogeneous, non-hematopoietic fibroblast-like cell with the potential for differentiating into connective tissue cells of multiple lineages. It plays a significant role in tissue repair and regeneration, and interacts with immune cells to downregulate the immune response and enable immunosuppression via the induction of tolerance.
Synonyms
  • Mesenchymal Stromal Cell
  • MSC
Source cell origin
Extraembryonic structure that connects the fetus to the placenta. It contains two arteries and one vein.
Synonyms
  • Umbilical Cord
  • UMBILICAL CORD
  • chorda umbilicalis
  • funiculus umbilicalis
  • umbilical cord
show more synonyms
Age of donor (at collection) neonate
Collected in 2021
Passage number reprogrammed 3

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
Yes
Derived under GMP?
Yes
Available as clinical grade?
Yes

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Essential 8™
Supplements
FGF 10 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: NCIT_C12706
In vitro spontaneous differentiation
Marker Expressed
GATA-4
Yes
Morphology
Mesoderm
Ont Id: NCIT_C12750
In vitro spontaneous differentiation
Marker Expressed
HAND1
Yes
Morphology
Ectoderm
Ont Id: NCIT_C12703
In vitro spontaneous differentiation
Marker Expressed
Otx2
Yes
Morphology

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46xx
Passage number: 12
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
SNP typing array
NGS (targeted sequencing)
Expanded cancer panel containing 264 cancer-associated genes.