HS480

General#

Cell Line

hPSCreg Name KIe026-A
Alternative name(s)
HS480
Cell line type Human embryonic stem cell (hESC)
Last update 7th October 2019
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Provider

Generator Karolinska Institutet (KI)
Derivation country Sweden

External Databases

Cellosaurus CVCL_C224

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information#

General Donor Information

Phenotype and Disease related information (Donor)

Diseases

Ethics#

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Regional ethical committee
Approval number

hESC Derivation#

Date of derivation 2006-11-28
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
Expansion status 6
Stephenson 2007
ICM morphology C
Stephenson 2007
Trophectoderm morphology g
Stephenson 2007
ZP removal technique Mechanical
Cell isolation Mechanical
Cell seeding Isolated ICM

Culture Conditions#

Feeder cells Human Foreskin Fibroblast
Cellfinder Ont Id: CELDA_000001419
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
glutamax
Serum Replacement 20
FGF-2

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)