Generation of the CTRL EiPS J9 mR6F-8 iPSC line derived from healthy human outgrowth blood endothelial cells (BOECs) using mRNA reprogramming methodology
Summary
mRNA reprogramming is a technology for generating iPSCs with high efficiency and safety. However, it is not suitable for reprogramming non-adherent cells, the primary cell type in blood. An alternative is to obtain adherent cells from blood for this technology. To validate this approach, we generated a human iPSC line from blood outgrowth endothelial cells (BOECs) using mRNA-based reprogramming. The resulting line, CTRL EiPS J9 mR6F-8, meets iPSC criteria, including an undifferentiated state, pluripotency, genome integrity. This line is available upon request. Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Selvitella S, Franco MP, Aran B, Veiga A, Kuebler B |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Dec;81:103606 |
| PubMed | 39541769 |
| DOI | 10.1016/j.scr.2024.103606 |