Generation and characterization of human-derived iPSC lines from one pair of dizygotic twins discordant for schizophrenia
Summary
Schizophrenia (SCZ) is a debilitating neurodevelopmental disorder with a high heritability. In this study, peripheral blood mononuclear cells (PBMCs) were donated by a pair of dizygotic twins. The female was clinically diagnosed as SCZ by Diagnostic and Statistical Manual of Mental Disorders IV (DSM-IV) criteria, and her unaffected male sibling was healthy control. Induced pluripotent stem cells (iPSCs) were established using Episomal vectors carrying reprograming factors OCT4, SOX2, NANOG, LIN28, c-MYC, KLF4, and SV40LT. These lines with normal karyotype highly expressed pluripotency markers and are capable to differentiate into derivatives of three germ layers. Both lines are negative of mycoplasma. Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Ni P, Wang H, Cai J, Ran J, Jiang Y, Zhao L, Wei J, Ni R, Wang Y, Ma X, Wang Q, Guo W, Li T |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 Apr;60:102710 |
| PubMed | 35182860 |
| DOI | 10.1016/j.scr.2022.102710 |