Generation and characterization of human-derived iPSC lines from one pair of dizygotic twins discordant for schizophrenia


Schizophrenia (SCZ) is a debilitating neurodevelopmental disorder with a high heritability. In this study, peripheral blood mononuclear cells (PBMCs) were donated by a pair of dizygotic twins. The female was clinically diagnosed as SCZ by Diagnostic and Statistical Manual of Mental Disorders IV (DSM-IV) criteria, and her unaffected male sibling was healthy control. Induced pluripotent stem cells (iPSCs) were established using Episomal vectors carrying reprograming factors OCT4, SOX2, NANOG, LIN28, c-MYC, KLF4, and SV40LT. These lines with normal karyotype highly expressed pluripotency markers and are capable to differentiate into derivatives of three germ layers. Both lines are negative of mycoplasma. Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Ni P, Wang H, Cai J, Ran J, Jiang Y, Zhao L, Wei J, Ni R, Wang Y, Ma X, Wang Q, Guo W, Li T
Journal Stem cell research
Publication Date 2022 Apr;60:102710
PubMed 35182860
DOI 10.1016/j.scr.2022.102710

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