LVIP04-LC12-1
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General
Cell Line |
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| hPSCreg name | LVPEIi006-B |
| Cite as: | LVPEIi006-B (RRID:CVCL_D6MZ) |
| Alternative name(s) |
LVIP04-LC12-1
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 5th March 2024 |
| Notes | This is a GMP grade, LCA12 patient-specific, induced pluripotent stem cell line, carrying a homozygous mutation in RD3 gene, at the exon 2 splice donor site. This mutation (c.296+1 G>A) results in splice skipping and inclusion of Intron 2, frameshift, and premature translational termination after codon 99. This iPSC line was generated by the reprogramming of donor skin-derived human dermal fibroblasts (HDFs) using xeno-free reagents and integration-free CTS™ CytoTune™-iPS 2.1 Sendai Reprogramming Kit (Invitrogen, A34546) containing Sendai viral vectors encoding the human OCT4, SOX2, KLF4 and L-Myc genes. This iPSC line maintained proper colony morphology, expressed the stemness and pluripotency markers OCT4, SOX2, NANOG, KLF4, SSEA4, and exhibited a normal karyotype at passage 15. This line efficiently differentiates into all three lineages and could generate retinal organoids and mature retinal pigmented epithelial cells. |
| User feedback | |
Provider |
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| Generator | L.V. Prasad Eye Institute (LVPEI) |
External Databases |
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| Cellosaurus | CVCL_D6MZ |
| Wikidata | Q127382713 |
General Information |
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hIPSC Derivation
General |
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