Generation of a transgene-free human induced pluripotent stem cell line (UNIPDi001-A) from oral mucosa epithelial stem cells
Summary
Human oral mucosa epithelial stem cells (hOMESCs) were obtained from a fresh oral biopsy collected from a healthy subject at the Fondazione Banca degli Occhi del Veneto (FBOV). An integration-free reprogramming protocol was applied exploiting episomal plasmids transfected into cells using a Nucleofector device. Around day 20 post transfection, several human induced pluripotent stem cell (hiPSC) colonies were manually picked and expanded. One of these (UNIPDi001-A-hiPSCs) expressed undifferentiated state marker alkaline phosphatase along with a panel of pluripotency state markers and was able to differentiate into the derivatives of all the three germ layers. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Alvisi G, Trevisan M, Masi G, Canel V, Caenazzo L, Nespeca P, Barzon L, Di Iorio E, Barbaro V, Palù G |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2018 Apr;28:177-180 |
| PubMed | 29547871 |
| DOI | 10.1016/j.scr.2018.02.007 |