Generation of a transgene-free human induced pluripotent stem cell line (UNIPDi001-A) from oral mucosa epithelial stem cells


Human oral mucosa epithelial stem cells (hOMESCs) were obtained from a fresh oral biopsy collected from a healthy subject at the Fondazione Banca degli Occhi del Veneto (FBOV). An integration-free reprogramming protocol was applied exploiting episomal plasmids transfected into cells using a Nucleofector device. Around day 20 post transfection, several human induced pluripotent stem cell (hiPSC) colonies were manually picked and expanded. One of these (UNIPDi001-A-hiPSCs) expressed undifferentiated state marker alkaline phosphatase along with a panel of pluripotency state markers and was able to differentiate into the derivatives of all the three germ layers. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Alvisi G, Trevisan M, Masi G, Canel V, Caenazzo L, Nespeca P, Barzon L, Di Iorio E, Barbaro V, Palù G
Journal Stem cell research
Publication Date 2018 Apr;28:177-180
PubMed 29547871
DOI 10.1016/j.scr.2018.02.007

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