ESIBIe005-A

HES-5

General#

Cell Line

hPSCreg Name ESIBIe005-A
Alternative name(s)
HES-5
Cell line type Human embryonic stem cell (hESC)
Last update 13th September 2017
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Provider

Generator ES Cell International Pte Ltd. (ESIBI)
Derivation country Singapore

External Databases

Cellosaurus CVCL_D095

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics#

We are currently revising this section.

hESC Derivation#

Cell isolation Immunosurgery

Culture Conditions#

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Passage method Enzymatically and Mechanically
Medium Other medium:
Base medium: High glucose DMEM
Main protein source: Fetal bovine serum
Serum concentration: 20 %
Supplements
FGF 2 %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3B
Yes
GABRB3
Yes
GDF3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
TDGF1
Yes
Alkaline Phosphatase
Yes
SSEA-1
Yes
SSEA-3
No
SSEA-4
No
TRA 1-60
Yes
TRA 1-81
No

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)