SLC4A1-21-C1

General

Cell Line

hPSCreg name MUSIi019-A
Cite as:
MUSIi019-A (RRID:CVCL_C0FI)
Alternative name(s)
SLC4A1-21-C1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 30th January 2023
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Provider

Generator Faculty of Medicine Siriraj Hospital (MUSI)

External Databases

Cellosaurus CVCL_C0FI
Wikidata Q112930147
BioSamples SAMEA112365036

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female
Ethnicity Thai

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Distal Renal Tubular Acidosis
Genetic variants
17q21.31
NM_000342.4:c.1331C>A
NP_000333.1:p.Thr444Asn
Heterozygous
SCV002522620
PMID: 36320073

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA112365035

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. N Rungroj, Siriraj Genomics, Mahidol University, Bangkok, Thailand
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Siriraj IRB
Approval number COA no. si 353/2021
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A sample of peripheral blood mononuclear cells.
Synonyms
  • Peripheral Blood Mononuclear Cell Specimen
  • PBMC Specimen
  • PBMC Sample
  • Peripheral Blood Mononuclear Cell Sample
  • Peripheral Blood Mononuclear Cell (PBMC)
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Morphology
AFP-1.tif
alpha fetoprotein (AFP)
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Morphology
betaIII-tubulin-1.tif
Beta III Tubulin

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Passage number: 20
Karyotyping method: G-Banding

Other Genotyping (Cell Line)