General

Cell Line
hPSCreg name	RCe011-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	RCe011-A (RRID:CVCL_C789)
Alternative name(s)	RC-7, RC7
Cell line type	Human embryonic stem cell (hESC)
Similar lines	No similar lines found.
Last update	28th November 2017
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Provider
Generator	Roslin Cells (RC) Contact: Derivation of Clinical Grade hES Cells
Owner	Derivation of Clinical Grade hES Cells
Distributors	Derivation of Clinical Grade hES Cells National Institute for Biological Standards and Control - UK Stem Cell Bank (USCB)
Derivation country	United Kingdom
External Databases
Cellosaurus	CVCL_C789
BioSamples	SAMEA104620772
Wikidata	Q54949408
General Information
Publications	De Sousa PA et al. Derivation of the human embryonic stem cell line RCe011-A (RC-7). Stem cell research. 2016 Mar;16(2):485-8. De Sousa PA et al. Development and production of good manufacturing practice grade human embryonic stem cell lines as source material for clinical application. Stem cell research. 2016 Sep;17(2):379-390.
* Is the cell line readily obtainable for third parties?	Yes

Donor Information

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases
External Databases (Donor)
BioSamples	SAMEA104620771

Ethics

Was the embryo established purely for research purposes?	No
Have both parents consented to the use of the embryo for ESC derivation?	Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived?	Yes
Was the consent voluntarily given?	Yes
Alternatives to consent are available?	Yes
Alternatives to consent
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised.	anonymised
Does consent explicitly allow the derivation of pluripotent stem cells?	Yes
Does consent expressly prevent the derivation of pluripotent stem cells?	No
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample?	No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	The Scotland A Research Ethics Committee/HEFA
Approval number	R0136

hESC Derivation

Date of derivation	2009-05-05
Embryo stage	Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?	Yes
PGD Embryo?	No
ZP removal technique	Mechanical
Cell isolation	None
Cell seeding	Isolated ICM

Culture Conditions

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
POU5F1 (OCT-4)	Yes
SSEA-4	Yes
SSEA-1	No

Microbiology / Virus Screening
HIV 1	Negative
HIV 2	Negative
Hepatitis B	Negative
Hepatitis C	Negative

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46XY Passage number: 10
Other Genotyping (Cell Line)