Generation of a gene-corrected human isogenic iPSC line from an Alzheimer's disease iPSC line carrying the London mutation in APP (V717I)
Summary
We report the genome-editing of an existing iPSC line carrying the London mutation in APP (V717I) into an iPSC line in which the pathogenic mutation was corrected. The resulting isogenic iPSC line maintained pluripotent stem cell morphology, a normal karyotype, expression of pluripotency markers and the ability to differentiate into the three germ-layers in vitro. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Hernández D, Morgan Schlicht S, Daniszewski M, Karch CM, Dominantly Inherited Alzheimer Network (DIAN), Goate AM, Pébay A |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 May;53:102373 |
| PubMed | 34088002 |
| PubMed Central | PMC10084742 |
| DOI | 10.1016/j.scr.2021.102373 |