HT977A

General

Cell Line

hPSCreg name TRNDi032-A
Cite as:
TRNDi032-A (RRID:CVCL_C9LN)
Alternative name(s)
HT977A
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
TRNDi036-A
(HT980A)
Donor diseases:
Alagille Syndrome
NCHi012-A
(NCH089)
Donor's gene variants:
JAG1
Donor diseases:
Alagille Syndrome
TRNDi038-A
(HT978A)
Donor diseases:
Alagille Syndrome
NCHi011-A
(NCH092)
Donor diseases:
Alagille syndrome
TRNDi037-A
(HT976A)
Donor diseases:
Alagille Syndrome
Last update 31st August 2023
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Provider

Generator NIH/NCATS-TRND Branch (TRND)

External Databases

BioSamples SAMEA112354454
Cellosaurus CVCL_C9LN
Wikidata Q123033648

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Alagille Syndrome
  • Arteriohepatic Dysplasia

External Databases (Donor)

BioSamples SAMEA112354455

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. gilbertma@chop.edu
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information gilbertma@chop.edu
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Patients were consented into an IRB-approved study at the Children’s Hospital of Philadelphia and the study team obtained written consent.
Approval number 92-000527
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
An immature lymphocyte that has enlarged in response to antigenic stimulation.
Synonyms
  • Lymphoblast
  • lymphoblast
  • lymphoblastic

Reprogramming method

Vector type Non-integrating
Vector Episomal

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Other medium:
Base medium: Stemflex
Main protein source:
Serum concentration: %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SOX2
Yes
TRA 1-81
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46

Other Genotyping (Cell Line)